PRODUÇÃO DE SORO HIPERIMUNE ANTIESTREPTOLISINA "O"

Abstract

Streptococcus pyogenes is a Gram-positive, B-hemolytic and group A cocci according to Lancefield's classification. It is an important pathogen causing suppurative diseases and non-suppurative complications. Among bacteria, S pyogenes is the most common cause of pharyngitis. These microorganisms have become an important target of studies nowadays due to their ability to produce serious and potentially fatal diseases when not identified and treated quickly. Because its transmission is through acrosols, places with agglomerations increase the opportunity for dissemination of this microorganism. It mainly caters to children between the ages of 5 and 15, but infants and adults are also susceptible. The detection of S. pyoge can be through biochemical, microbiological and immunological techniques. The aim of the present work was the production of antistreptolysin "O hyperimmune serum (ASLO) for the diagnosis of recent infection caused by Spyogenes. For the production of the antiserum, 2 male New Zealand rabbits were immunized by subcutaneous injections. turbidimetry and dual immunodiffusion techniques, specificity and antibody concentration were analyzed, where it was possible to conclude that the animals had an immune response with a titer of 14.4 mi (cobain 011c 19, II/ml (guinea pig 02).