ESTUDO DO CRESCIMENTO DO MELANOMA MURINO EM CAMUNDONGOS C57BL/6 QUANDO DESAFIADOS POR DUAS VIAS DE TRANSPLANTES CELULARES

Abstract

Cancers, today, are among the causes of the greatest number of deaths worldwide. Malignant melanoma is a relatively common neoplasm that, not so long ago, was considered almost uniformly fatal. The present study aims to observe the difference in the development of murine melanoma, as well as the survival of animals when two different inoculation routes are used. The experiment consisted of inoculating tumor cells, the B16F10, by subcutaneous and intravenous routes, in C57BL/6 mice, aiming to analyze and verify the difference in the development of subcutaneous tumors and the formation of lung metastases. The study is based on the injection of tumor cells in the animals' back so that there is the development of the subcutaneous tumor and on their transplantation, in the tail vein of the mice, so that the formation of metastases occurs. In the two phases of the experiment, the C57BL/6 mice separated into groups received different doses. It was observed that the formation of subcutaneous tumors is dose-dependent, and that animal survival is directly related to the dose of inoculated cells. The presence of metastases in the lungs occurred only in the lungs, reinforcing literature data that show that the B16F10 cell line, when inoculated in the tail vein of C57BL/6 mice, only colonizes this organ.